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This compares favourably with the IRES activity of the cellular IRESs found in Apaf1 and cmyc (nine and 50 times respectively) and the viral IRESs found in HRV and EMCV (eight and 14 times) when present in the same assay system (Figure 2b). There is a slight downregulation of expression of the upstream Renilla cistron in the vector pRBF (to about 70% of the control). This has been observed in other IREScontaining dicistronic transcripts and most likely reflects a competition between capdependent and IRESdependent translation on the same transcript.
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